EFFECTS OF THE STRUCTURE OF POLY(VINYL ALCOHOL) ON THE DEHYDROGENATION REACTION BY POLY(VINYL ALCOHOL) DEHYDROGENASE FROM PSEUDOMONAS SP 113PS

被引：21
|
作者：
HATANAKA, T
KAWAHARA, T
ASAHI, N
TSUJI, M
机构：
[1] Kuraray Co., Ltd., Kurashiki, Okayama 710
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 07期
关键词：
D O I：
10.1271/bbb.59.1229
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Poly(vinyl alcohol) dehydrogenase (PVADH-S) purified from Pseudomonas sp, 113P3 dehydrogenized poly(vinyl alcohol) (PVA), but the enzyme reaction was significantly affected by changing the properties of PVA, in terms of saponification degree, ethylene content, polymerization degree, tacticity, and 1,2-glycol content. The apparent V-max/K-m value of PVA containing 10 mol% ethylene was over 10-fold greater than that of PVA. The hydrophobicity of PVA-related polymers might be important in the affinity for the PVADH-S.
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页码：1229 / 1231
页数：3
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