AN EXAMPLE OF THE ISOLATION OF MINOR COMPONENTS FROM MULTICOMPONENT MIXTURES

Several examples are presented to illustrate a methodology for isolating minor components in multicomponent mixtures by high performance liquid chromatography. When the minor component is in small amount and elutes in front of a major component, displacement effects can be utilized to increase the sample size and the concentration of the minor component as a first enrichment step. When the minor component is in reasonably large amount, appropriate choice of the cut points can lead to purification in one step by loading until touching bands. Use of columns greater than analytical size can reduce significantly the number of repetitive injections required to produce a few milligrams of substance and to increase the production rate.