LOCAL AND SYSTEMIC ANTIBODY-RESPONSE IN INFANTS AFTER ORAL-ADMINISTRATION OF INACTIVATED ENTEROPATHOGENIC ESCHERICHIA-COLI SEROTYPE O111 AND O55

In ten infants divided into two groups (up to one month of age and at 2-7 months of age) the dynamics and formation of different antibody isotypes produced locally in the intenstine and in serum after orally administered inactivated enteropathogenic E. coli strains O111 and O55 was followed during 30 d after the first and booster dose by using an indirect immunofluorescence method. Infants up to one month of age produced antibodies of IgM isotype in stool together with the IgA isotype after the first and booster dose of the vaccine against both antigens. Serum IgG antibody increased after 2 d following the first and second dose of antigens and remained higher during 5 d. The infants aged 2-7 months expressed predominantly the IgA isotype response in stool after the first and booster dose of antigens. The serum immunoglobulin levels did not change after oral antigen administration. © 1990 Institute of Microbiology, Academy of Sciences of the Czech Republic.