REGULATION OF ALPHA-AMYLASE FORMATION IN ASPERGILLUS-ORYZAE AND ASPERGILLUS-NIDULANS TRANSFORMANTS

被引：28
|
作者：
LACHMUND, A [1 ]
URMANN, U [1 ]
MINOL, K [1 ]
WIRSEL, S [1 ]
RUTTKOWSKI, E [1 ]
机构：
[1] TH DARMSTADT, INST BIOCHEM, W-6100 DARMSTADT, GERMANY
来源：
CURRENT MICROBIOLOGY | 1993年 / 26卷 / 01期
关键词：
D O I：
10.1007/BF01577242
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aspergillus oryzae was grown in a minimal medium supplemented with nine different carbon sources. Alpha-Amylase formation was induced by starch and maltose. With glucose, no carbon catabolite repression was observed, but surprisingly alpha-amylase production was induced; all other tested carbon sources failed to influence alpha-amylase synthesis. Simultaneous cultivation with two different carbon sources confirmed this alpha-amylase regulation. Northern blot analysis showed that alpha-amylase gene expression was controlled at the level of transcription. For further characterization of alpha-amylase expression, Aspergillus nidulans was transformed by the amyl gene of A. oryzae. The regulation of alpha-amylase formation in three analyzed transformants by different carbon sources was comparable to that of the A. oryzae wildtype.
引用
收藏
页码：47 / 51
页数：5
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