THE SPOOA PROTEIN OF BACILLUS-SUBTILIS IS A REPRESSOR OF THE ABRB GENE

被引：307
|
作者：
STRAUCH, M [1 ]
WEBB, V [1 ]
SPIEGELMAN, G [1 ]
HOCH, JA [1 ]
机构：
[1] UNIV BRITISH COLUMBIA, DEPT MICROBIOL, VANCOUVER V6T 1W5, BC, CANADA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 05期
关键词：
Footprinting; Sporulation; Transcription;
D O I：
10.1073/pnas.87.5.1801
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The spoOA gene of Bacillus subtilis is critical for the initial stages in the developmental cycle leading to the formation of an endospore. We show that one function of the SpoOA protein is to negatively regulate another regulatory locus, abrB, which controls the expression of many genes associated with the onset of sporulation. Purified SpoOA protein binds to a specific region of the abrB promoter and functions as a repressor of transcription in an in vitro assay. The binding of the SpoOA protein is independent of the binding of the AbrB protein, which is known to autoregulate its expression. This independence mirrors the temporal sequence of events in abrB control.
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页码：1801 / 1805
页数：5
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