Methanol extract of mangosteen peel (Garcinia mangostana L.) increase activity acetylcholinesterase and glutathione peroxidase and reduce MDA in diazinon exposed rat

Introduction: A study has been conducted on the methanol extract of mangosteen peel (MEMP) in rats exposed to diazinon pesticides (DZN). The purpose of this study is to prove that the methanol extract of mangosteen peel can increase the activity of AChE and GPx as well as decrease MDA concentration of rats that were exposed to DZN. Method: Thirty healthy male Wistar rats divided into five groups. The control group (K-), group II was exposed to DZN (K+), group Ill was exposed to DZN and given 200 mg/kg MEMP (P1), group IV was exposed to DZN and given 400 mg/kg MEMP (P2) and group V was exposed to DZN and given 600 mg/kg MEMP (P3), and performed for three weeks. Blood samples were taken for analysis of acetylcholinesterase (AChE) activity, glutathione peroxidase (GPx) activity, and MDA levels. Results: The results analyzed by ANOVA test followed by post hoc test. MEMP treatment showed significantly higher AChE activity (p < 0.005) than exposed without MEMP. Compared with control group, the greatest increase is in P2 (1105.26 +/- 1.53 U/L); GPx activity was also significantly higher (p < 0.005) between the exposed treatment 990.00 +/- 47.43 with P2 (1236.00 +/- 12.76) and P3 (1530.00 +/- 58.74). MDA levels were significantly lower (p < 0.005) among the three treatments were exposed to the P1, P2, and P3, treatment gave the highest decrease in MDA is P2. Conclusions: The conclusion is MEMP can increase activity of AChE, GPx and decrease MDA concentration on rats exposed to DZN.