A COMPARISON OF THE CLEARANCE OF OVINE AND HUMAN CORTICOTROPIN-RELEASING HORMONE (CRH) IN MAN AND SHEEP - A POSSIBLE ROLE FOR CRH-BINDING PROTEIN

被引:48
|
作者
SAPHIER, PW
FARIA, M
GROSSMAN, A
COY, DH
BESSER, GM
HODSON, B
PARKES, M
LINTON, EA
LOWRY, PJ
机构
[1] ST BARTHOLOMEWS HOSP,COLL MED,DEPT ENDOCRINOL,LONDON EC1A 7BE,ENGLAND
[2] TULANE UNIV,SCH MED,DEPT MED,NEW ORLEANS,LA 70118
基金
英国惠康基金;
关键词
D O I
10.1677/joe.0.1330487
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A specific binding protein for human corticotrophin-releasing hormone (hCRH), which does not bind to the ovine hormone (oCRH), has recently been demonstrated in human plasma. No such binding protein has been found in sheep plasma. We have investigated the half-life of human and ovine CRH in man and in sheep. Peptides were measured directly in plasma with two-site immunoradiometric assays, as these assays are unaffected by the presence of inactivated peptide fragments. In man, the half-life of hCRH (30.5+/-3.3 min; mean +/- S.E.M.) Was significantly (P<0.001) less than that of oCRH (42.8+/-6.4 min). In sheep, there was no significant difference between the half-life of hCRH (46.5+/-7.2 min) and that of oCRH (39.8+/-10.1 min); these half-lives were also significantly (P<0.001) longer than that of hCRH in man. One possible explanation for the shorter half-life of hCRH in man is that the clearance of hCRH is enhanced by CRH-binding protein, although other binding proteins often have the opposite effect. Peak ACTH and cortisol responses occurred earlier in sheep than in man, although no differences were found in the response times to oCRH or hCRH within either species. The responses were more sustained in sheep than in man, and the previously reported biphasic response was only seen in some of the sheep and not in man. Absolute responses to either peptide were greater in sheep than in man; however, in man an 8.1-fold rise in ACTH was measured in response to oCRH, while hCRH gave a significantly (P=0.043) smaller 4.4-fold response. These experiments are discussed with particular reference to the effect of hCRH-binding protein and its possible role in potentiating the clearance of the peptide.
引用
收藏
页码:487 / 495
页数:9
相关论文
共 50 条
  • [1] Aging associated changes in amygdalar corticotropin-releasing hormone (CRH) and CRH-binding protein in Fischer 344 rats
    Xiao, C
    Sartin, J
    Mulchahey, JJ
    Segar, T
    Sheriff, S
    Herman, JP
    Kasckow, JW
    BRAIN RESEARCH, 2006, 1073 : 325 - 331
  • [2] Blunted ACTH and cortisol responses to systemic injection of corticotropin-releasing hormone (CRH) in fibromyalgia role of somatostatin and CRH-binding protein
    Riedel, W
    Schlapp, U
    Leck, S
    Netter, P
    Neeck, G
    NEUROENDOCRINE IMMUNE BASIS OF THE RHEUMATIC DISEASES II, PROCEEDINGS, 2002, 966 : 483 - 490
  • [3] HORSE PLASMA CORTICOTROPIN-RELEASING HORMONE (CRH) - CHARACTERIZATION AND LACK OF A LATE GESTATIONAL RISE OR A PLASMA CRH-BINDING PROTEIN
    ELLIS, MJ
    LIVESEY, JH
    DONALD, RA
    JOURNAL OF ENDOCRINOLOGY, 1994, 143 (03) : 455 - 460
  • [4] GLYCOSYLATION OF THE MOUSE CORTICOTROPIN-RELEASING HORMONE-BINDING PROTEIN IS REQUIRED FOR MAXIMAL CRH-BINDING ACTIVITY
    CORTRIGHT, DN
    SEASHOLTZ, AF
    FASEB JOURNAL, 1995, 9 (06): : A1361 - A1361
  • [5] Systemic adrenocorticotropic hormone administration down-regulates the expression of corticotropin-releasing hormone (CRH) and CRH-binding protein in infant rat hippocampus
    Wang, W
    Murphy, B
    Dow, KE
    Andrew, RD
    Fraser, DD
    PEDIATRIC RESEARCH, 2004, 55 (04) : 604 - 610
  • [6] Systemic Adrenocorticotropic Hormone Administration Down-Regulates the Expression of Corticotropin-Releasing Hormone (CRH) and CRH-Binding Protein in Infant Rat Hippocampus
    Wei Wang
    Bridgid Murphy
    Kimberly E Dow
    R David Andrew
    Douglas D Fraser
    Pediatric Research, 2004, 55 : 604 - 610
  • [7] CORTICOTROPIN-RELEASING HORMONE (CRH)-BINDING PROTEIN INTERFERENCE WITH CRH ANTIBODY-BINDING - IMPLICATIONS FOR DIRECT CRH IMMUNOASSAY
    LINTON, EA
    PERKINS, AV
    HAGAN, P
    POOLE, S
    BRISTOW, AF
    TILDERS, F
    CORDER, R
    WOLFE, CDA
    JOURNAL OF ENDOCRINOLOGY, 1995, 146 (01) : 45 - 53
  • [8] CORTICOTROPIN-RELEASING HORMONE (CRH) IS A RESPIRATORY STIMULANT IN HUMANS - A COMPARATIVE-STUDY OF HUMAN AND OVINE CRH
    NINK, M
    SALOMON, E
    COUTINHO, M
    TREESE, N
    BERNHARD, G
    KRAUSE, U
    BEYER, J
    LEHNERT, H
    LIFE SCIENCES, 1994, 54 (23) : 1793 - 1799
  • [9] CORTICOTROPIN-RELEASING HORMONE (CRH)-BINDING PROTEIN - REDUCTION IN THE ADRENOCORTICOTROPIN-RELEASING ACTIVITY OF PLACENTAL BUT NOT HYPOTHALAMIC CRH
    LINTON, EA
    BEHAN, DP
    SAPHIER, PW
    LOWRY, PJ
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1990, 70 (06): : 1574 - 1580
  • [10] Corticotropin-releasing hormone (CRH) - Editorial
    Kasckow, J
    PEPTIDES, 2001, 22 (05) : 703 - 703