FLOW-CYTOMETRIC QUANTIFICATION OF CD34-POSITIVE CELLS

被引:0
|
作者
TICHELLI, A
KRALJ, A
HUXOL, H
SCHONENBERGER, A
RABAGLIO, M
BOREL, J
MULLER, R
BARGETZI, M
GRATWOHL, A
SIGNER, E
SPECK, B
机构
[1] KANTONSSPITAL BASEL,DEPT INNERE MED,HAMATOL ABT,BASEL,SWITZERLAND
[2] UNIV BASEL,KANTONSSPITAL,DEPT ZENT LAB,HAMATOL LAB,CH-4031 BASEL,SWITZERLAND
[3] UNIV BASEL,KINDERSPITAL,BASEL,SWITZERLAND
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中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In the present study three clinical applications of flow-cytometric quantification of CD34-positive cells in the field of blood and marrow transplantation are evaluated. By daily determination of CD34-positive cells in the peripheral blood after mobilization of stem cells with chemotherapy or growth-factors it is possible to set the optimal time for collection of the transplant material. Thereafter the yield of CD34-positive cells within the harvested material is determined. Finally, the efficacy of positive CD34 selection using a biotin-avidin column is evaluated. Initial experience is presented here.
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页码:400 / 404
页数:5
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