TENASCIN EXPRESSION IN HUMAN GLIOMA CELL-LINES AND NORMAL-TISSUES

被引：72

作者：

VENTIMIGLIA, JB

WIKSTRAND, CJ

OSTROWSKI, LE

BOURDON, MA

LIGHTNER, VA

BIGNER, DD

机构：

[1] DUKE UNIV, MED CTR, DEPT PATHOL, BOX 3156, DURHAM, NC 27710 USA

[2] DUKE UNIV, MED CTR, DEPT MED, DURHAM, NC 27710 USA

[3] DUKE UNIV, MED CTR, DEPT CELL BIOL, DURHAM, NC 27710 USA

[4] DUKE UNIV, MED CTR, PREUSS LAB BRAIN TUMOR RES, DURHAM, NC 27710 USA

[5] CALIF INST BIOL RES, LA JOLLA, CA 92037 USA

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1992年 / 36卷 / 01期

关键词：

TENASCIN; EXTRACELLULAR MATRIX; GLIOMA; ISOFORMS; ALTERNATIVE MESSENGER RNA SPLICING; IMMUNOTHERAPY;

D O I：

10.1016/0165-5728(92)90029-K

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Tenascin expression was evaluated in 21 human glioma cell lines and in normal adult tissue extracts by Western and Northern blotting. The cell lines differed in their relative expression of tenascin in the cell-associated and supernatant compartments. Glioma cell line tenascin production was not uniformly stimulated by changes in fetal bovine serum concentration in the growth media. In most glioma cell lines and normal tissue extracts, reducing Western blots and Northern blots revealed two tenascin species, respectively: a major 340 kDa polypeptide and a 9 kb RNA transcript accompanied by a less intense 250 kDa polypeptide and 7 kDa RNA species. In U-87 MG and in normal adult kidney extracts, however, the 250 kDa band and 7 kb transcript were more prominent. Quantitation of tenascin in the glioma lines revealed variable levels that were significantly higher than those in the tissue extracts.

引用

页码：41 / 55

页数：15

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