PURIFICATION, CHARACTERIZATION AND CRYSTAL-GROWTH OF SAPORINS

被引：0

作者：

HUANG, DH ^{[1
]}

QIU, W ^{[1
]}

FU, ZJ ^{[1
]}

YE, XM ^{[1
]}

PAN, KZ ^{[1
]}

机构：

[1] ACAD SINICA, FUJIAN INST RES STRUCT MATTER, FUZHOU 350002, PEOPLES R CHINA

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 1995年 / 27卷 / 03期

关键词：

SAPORINS; RIBOSOME-INACTIVATING PROTEIN; PURIFICATION; CRYSTAL GROWTH;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three saporins were separated and purified from the seed extracts of Saponaria officinalis by precipitation at 40 similar to 100% saturation of(NH4)(2)SO4, and CM-Sepharose and CM-cellulose column chromatography. Their molecular weights are about 29500 and pI values higher than 9.5. They are single-chain ribosome-inactivating proteins(RIPs) and have potent activity of inhibiting pro-rein synthesis. After screening a large number of conditions for growing, the protein crystals for two of them were obtained.

引用

页码：333 / 336

页数：4

共 7 条

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