DENSITOMETRIC DETERMINATION OF CONJUGATED ESTROGENS IN THE RAW-MATERIAL AND IN PHARMACEUTICAL PREPARATIONS

被引：9
|
作者：
NOVAKOVIC, J [1 ]
AGBABA, D [1 ]
VLADIMIROV, S [1 ]
ZIVANOVSTAKIC, D [1 ]
机构：
[1] FAC PHARM BELGRADE, DEPT PHARMACEUT CHEM, POB 146, DR SUBOTICA 8, YU-11000 BELGRADE, YUGOSLAVIA
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 03期
关键词：
Conjugated oestrogens; densitometry; quantitative assay; raw material; sample preparation; TLC separation;
D O I：
10.1016/0731-7085(90)80034-M
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A densitometric method for determination of complex mixtures of conjugated oestrogens in raw material and tablets was developed. The proposed procedure comprised hydrolysis of sodium sulphate esters of oestrone, equilin, 17α-oestradiol, equilenin, 17α-dihydroequilin and 17α-dihydroequilenin, the chloroform extraction of free oestrogens and methyltestosterone (internal standard) and separation on TLC plates using chloroform-cyclohexane-dioxane-triethyl-amine (4.5:4.1:0.6, v/v) as the solvent for development. Quantitative assay was achieved by direct scanning of the oestrogen spots at 280 nm. The proposed method is simple, rapid, reproducible and adequate to control the content of conjugated oestrogens in the raw material and in pharmaceutical preparations. © 1990.
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页码：253 / 257
页数：5
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