THE INFLUENCE OF MESSENGER-RNA SECONDARY STRUCTURE ON EXPRESSION OF ENDOGLUCANASE IN BACILLUS-SUBTILIS

被引：3
|
作者：
LEE, BR [1 ]
KIM, JH [1 ]
PACK, MY [1 ]
机构：
[1] KAIST,DEPT BIOTECHNOL,TAEJON 305701,SOUTH KOREA
来源：
BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 10期
关键词：
D O I：
10.1007/BF01022392
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A gene for endoglucanase of Bacillus subtilis has been inserted into a Bacillus expression plasmid containing a strong BJ27 promoter and a synthetic ribosome binding site. Secondary structure analysis of mRNA showed the presence of a strong hairpin loop burying the SD sequence and the initiation codon. Alteration of secondary structure at this site by deletion analysis revealed a correlation between endoglucanase expression and accessibility of the ribosome binding site. Elimination of secondary structures increased endoglucanase expression over five-fold to a level at which endoglucanase occupied 60% of total protein which was secreted into culture medium.
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页码：1001 / 1006
页数：6
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