DETERMINATION OF RETINOID ACTIVITY BY AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY

被引：25
|
作者：
MICHEL, S [1 ]
COURSEAUX, A [1 ]
MIQUEL, C [1 ]
BERNARDON, JM [1 ]
SCHMIDT, R [1 ]
SHROOT, B [1 ]
THACHER, SM [1 ]
REICHERT, U [1 ]
机构：
[1] TEXAS A&M UNIV SYST,DEPT MED BIOCHEM & GENET,COLLEGE STN,TX 77843
来源：
ANALYTICAL BIOCHEMISTRY | 1991年 / 192卷 / 01期
关键词：
D O I：
10.1016/0003-2697(91)90213-D
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In normal human keratinocytes, retinoic acid suppresses the expression of the plasma membrane associated enzyme transglutaminase (TGm) at the pretranslational level. This finding led us to develop an enzyme-linked immunosorbent assay (ELISA) for the evaluation of biological activity of retinoids, i.e., natural and synthetic derivatives of vitamin A. In this assay, keratinocytes are cultured in a 96-well cluster in the presence of different retinoid concentrations. The expression of TGm is then quantified, without any extraction or purification step, using a TGm-specific monoclonal antibody and a peroxidase-conjugated secondary antibody. The dose-response curves obtained show this ELISA to be a sensitive and reproducible assay to determine the potency of retinoids. © 1991.
引用
收藏
页码：232 / 236
页数：5
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