MONOCLONAL-ANTIBODIES AFFECTING SPERM-ZONA BINDING AND OR ZONA-INDUCED ACROSOME REACTION

被引：24

作者：

FANN, CH ^{[1
]}

LEE, CYG ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT OBSTET & GYNECOL,ANDROL LAB,VANCOUVER V6T 2B5,BC,CANADA

来源：

JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 1992年 / 21卷 / 02期

基金：

英国医学研究理事会;

关键词：

ANTISPERM MONOCLONAL ANTIBODIES; INVITRO FERTILIZATION; SPERM-ZONA BINDING; ZONA-INDUCED ACROSOME REACTION;

D O I：

10.1016/0165-0378(92)90023-W

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The majority of anti-sperm monoclonal antibodies which were shown to inhibit in vitro fertilization in our laboratory react with antigens in the acrosomal region of spermatozoa. To elucidate the mechanism of human and/or mouse fertilization inhibition, the effects of these antibodies on mouse sperm-zona binding and mouse zona-induced acrosome reaction in vitro were studied. Among these antibodies, MS-4 and MS-7 were shown to react partially with uncapacitated mouse sperm, and to react more with capacitated mouse sperm, whereas HS-9, HS-11 and HS-63 react only with capacitated and acrosome-intact human or mouse sperm. HS-63 and MS-4 were shown to inhibit zona-induced acrosome reaction significantly, but not the sperm-zona binding. On the other hand, HS-9, HS-11 and MS-7 were shown to inhibit both sperm-zona binding and zona-induced acrosome reaction. The results of this study suggest that fertilization inhibition caused by these antibodies could result from that of the initial sperm-zona binding and/or zona-induced acrosome reaction.

引用

页码：175 / 187

页数：13

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