NEW CLASSES OF STREPTOMYCES-COELICOLOR-A3(2) MUTANTS BLOCKED IN UNDECYLPRODIGIOSIN (RED) BIOSYNTHESIS

被引：17
|
作者：
COCO, EA [1 ]
NARVA, KE [1 ]
FEITELSON, JS [1 ]
机构：
[1] AMER CYANAMID CO,DIV MED RES,PEARL RIVER,NY 10965
来源：
MOLECULAR & GENERAL GENETICS | 1991年 / 227卷 / 01期
关键词：
RECOMBINANT DNA; ANTIBIOTIC PRODUCTION; GENETIC ORGANIZATION; DIRECTED MUTANT SCREEN; PRODIGIOSIN;
D O I：
10.1007/BF00260702
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fifteen mutants of Streptomyces coelicolor A3(2) blocked in both the bipyrrole branch (redA) and a second site specific to the undecylprodigiosin pathway were characterized. Some of the mutants were ordered biosynthetically based on cosynthesis experiments. Complementation of each of the mutants with wild-type DNA cloned in low- and high-copy number plasmid vectors allowed the mutants to be separated into 12 new classes which are physically clustered within approximately 37 kb on the S. coelicolor genome. Early-step biosynthetic genes are centrally located and are flanked by later-step and regulatory genes.
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页码：28 / 32
页数：5
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