PLASMID PROFILES AND CURING OF PLASMIDS IN LACTOBACILLUS-PLANTARUM STRAINS ISOLATED FROM GREEN OLIVE FERMENTATIONS

被引：92
|
作者：
RUIZBARBA, JL [1 ]
PIARD, JC [1 ]
JIMENEZDIAZ, R [1 ]
机构：
[1] INRA,UNITE MICROBIOL,RECH LAITIERES STN,F-78350 JOUY EN JOSAS,FRANCE
来源：
JOURNAL OF APPLIED BACTERIOLOGY | 1991年 / 71卷 / 05期
关键词：
D O I：
10.1111/j.1365-2672.1991.tb03810.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Plasmid profiles of 35 Lactobacillus plantarum strains isolated from different green olive fermentors were obtained. A large number of plasmids in the CCC form (from 5 to 16) were present in all the tested strains as confirmed by a second dimension electrophoresis of DNA. These plasmids, all of which remain cryptic, ranged from 2-0 to 68 kb in size. Novobiocin, sodium dodecyl sulphate and ethidium bromide were used as plasmid-curing agents but only novobiocin induced loss of extrachromosomal DNA at a high frequency in these strains.
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页码：417 / 421
页数：5
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