LONG-TERM CRYOPRESERVATION OF TOXOPLASMA-GONDII

被引：0
|
作者：
POPESCU, C [1 ]
STERIU, D [1 ]
机构：
[1] FIRST CANTACUZINO INST,BUCHAREST,ROMANIA
来源：
CRYO-LETTERS | 1993年 / 14卷 / 06期
关键词：
CRYOPRESERVATION; TOXOPLASMA GONDII; DMSO; PROPAN-1; 2-DIOL SORBITOL; L-GLUTAMINE;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Optimal cryoprotection conditions for Toxoplasma gondii were investigated. The highest level of cell survival was obtained with a continuous slow cooling of 0.2-0.3 degrees C min(-1) from 24 degrees C to 20 degrees C and 1 degrees C min(-1) to -60 degrees C followed by immersion in liquid nitrogen with 8-10% (v/v) Me(2)SO in RPMI 1640 medium, supplemented with 20 mM Glutamine and fast thawing in 0.5 M Sorbitol at 37 degrees C
引用
收藏
页码：383 / 388
页数：6
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