EVALUATION OF A ONE TUBE REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION FOR THE DETECTION OF RUMINANT PESTIVIRUSES

被引：13
|
作者：
EASTON, LA
VILCEK, S
NETTLETON, PF
机构：
[1] MOREDUN RES INST,EDINBURGH EH17 7JH,MIDLOTHIAN,SCOTLAND
[2] UNIV VET MED,DEPT INFECT DIS & TROP VET MED,KOSICE 04181,SLOVAKIA
来源：
JOURNAL OF VIROLOGICAL METHODS | 1994年 / 50卷 / 1-3期
关键词：
PESTIVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RTTH DNA POLYMERASE;
D O I：
10.1016/0166-0934(94)90189-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A 'one tube' reverse transcription-polymerase chain reaction ('one tube' RT-PCR) using rTth DNA polymerase was compared with an existing RT-PCR using Taq DNA polymerase (Taq RT-PCR) to detect ruminant pestiviruses in infected cell cultures. The technically simpler and more convenient 'one tube' method was relatively insensitive detecting only 11 of the 34 samples tested, all of which were positive by Taq RT-PCR.
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页码：343 / 348
页数：6
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