SEX IDENTIFICATION BY POLYMERASE CHAIN-REACTION USING A Y-AUTOSOME HOMOLOGOUS PRIMER SET

被引:1
|
作者
IIDA, T [1 ]
NAKAHORI, Y [1 ]
TANAKA, K [1 ]
TSUTSUMI, O [1 ]
TAKETANI, Y [1 ]
NAKAGOME, Y [1 ]
机构
[1] UNIV TOKYO,FAC MED,DEPT OBSTET & GYNECOL,BUNKYO KU,TOKYO 113,JAPAN
来源
JAPANESE JOURNAL OF HUMAN GENETICS | 1993年 / 38卷 / 04期
关键词
POLYMERASE CHAIN REACTION; PCR; SEX IDENTIFICATION; SEQUENCE TAGGED SITE; STS;
D O I
10.1007/BF01907990
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We have developed a one-step polymerase chain reaction (PCR) technique which detects a sequence on the human Y chromosome and an autosomal sequence in one reaction. The method is very reliable for the sex determination, as the detection of the autosome-specific signal ensures the presence of DNA in the specimen even in the absence of the Y-specific signal.
引用
收藏
页码:429 / 431
页数:3
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