IMMUNOHISTOLOGICAL DETECTION OF SARUPLASE (RECOMBINANT SINGLE-CHAIN UROKINASE-TYPE PLASMINOGEN-ACTIVATOR) IN NORMAL RAT-TISSUE

被引：0
|
作者：
WOHRMANN, T
MATTHIESEN, T
BEIER, H
FLOHE, L
机构：
[1] Division Toxicology/Pathology, Research Centre Gruenenthal GmbH, Aachen, P. O. Box
来源：
HISTOCHEMISTRY | 1991年 / 96卷 / 02期
关键词：
D O I：
10.1007/BF00315988
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Saruplase - a recombinant single-chain urokinase-type plasminogen activator was identified immunohistochemically in normal rat tissue after intravenous administration by means of a polyclonal antibody. For this purpose, rat tissues were fixed in various ways (liquid nitrogen, ethanol, formaldehyd solution). Saruplase could be detected by the PAP method, streptavidinbiotin system and indirect immunofluorescence in the kidney (proximal tubule), liver (hepatocytes, Kupffer cells) and spleen (reticular cells). Saruplase was not localized in the rat endothelium. It is discussed that the rat-specific receptors for urokinase-type plasminogen activator on endothelial cells cannot bind Saruplase due to the extreme species specificity.
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页码：163 / 167
页数：5
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