ISOLATION OF CATHARANTHUS ALKALOIDS BY SOLID-PHASE EXTRACTION AND SEMIPREPARATIVE HPLC

被引：4

作者：

NAARANLAHTI, T ^{[1
]}

NORDSTROM, M ^{[1
]}

LAPINJOKI, SP ^{[1
]}

HUHTIKANGAS, A ^{[1
]}

LOUNASMAA, M ^{[1
]}

机构：

[1] TECH UNIV HELSINKI, ORGAN & BIOORGAN CHEM LAB, SF-02150 ESPOO, FINLAND

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1990年 / 28卷 / 04期

关键词：

Cell culture--Plant;

D O I：

10.1093/chromsci/28.4.173

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A combination of moderate-pressure chromatography on C18sorbent and preparative HPLC is developed for rapid isolation of alkaloids from Catharanthus roseus. The procedure is optimized for vindoline and catharanthine with respective yields of 3 and 2 mg per 1 g of dried leaves of the plant. The methodology is also applied for identification of the above and other alkaloids from cultured plant cells. © 1990 Oxford University Press.

引用

页码：173 / 174

页数：2

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