A MULTIPLEX POLYMERASE CHAIN-REACTION FOR DISTINGUISHING MYCOBACTERIUM-TUBERCULOSIS FROM MYCOBACTERIUM-TUBERCULOSIS COMPLEX

被引：14
|
作者：
SINCLAIR, K [1 ]
CHALLANS, JA [1 ]
KAZWALA, RR [1 ]
HEWINSON, RG [1 ]
SHARP, JM [1 ]
机构：
[1] CENT VET LAB,WEYBRIDGE,SURREY,ENGLAND
来源：
MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 05期
关键词：
MYCOBACTERIUM-TUBERCULOSIS COMPLEX; POLYMERASE CHAIN REACTION; DIAGNOSIS;
D O I：
10.1016/S0890-8508(95)91524-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40. (C) 1995 Academic Press Limited
引用
收藏
页码：291 / 295
页数：5
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