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A METHOD FOR DETECTION OF HUMAN PAPILLOMAVIRUS DNA IN STORED SLIDES OF STAINED CERVICAL SMEARS
被引:0
|作者:
SCARPA, A
[1
]
POZZI, G
[1
]
MANFRIN, E
[1
]
LANNUCCI, A
[1
]
BONETTI, F
[1
]
FONTANA, R
[1
]
FIOREDONATI, L
[1
]
机构:
[1] UNIV VERONA, IST MICROBIOL, I-37134 VERONA, ITALY
关键词:
Cervical cancer;
DNA Hybridization;
Epidemiology;
Papillomavirus;
D O I:
10.1016/S0344-0338(11)80231-1
中图分类号:
R36 [病理学];
学科分类号:
100104 ;
摘要:
A simple method was developed for extraction of DNA from stored slides of Papanicolaou stained cervical smears. HPV type 16 DNA was detected by DNA hybridization in 7/10 samples from known HPV-16 positive patients, whereas the 10 samples from negative patients were also negative with our assay. Using our method for obtaining data from stained cervical smears, it will be possible to design and perform historical cohort studies on stored material. © 1990, Gustav Fischer Verlag · Stuttgart · New York. All rights reserved.
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页码:666 / 667
页数:2
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