HISTAMINE-H3 RECEPTORS ARE NOT INVOLVED IN THE REGULATION OF RAT GASTRIC-SECRETION

The effects of histamine H-3 receptor activation [(R)alpha-methylhistamine] and blockade (thioperamide) on rat gastric secretion were determined in vivo and in vitro. (R)alpha-Methylhistamine (0.1-5-mu-mol/kg i.p.) did not modify secretory volume and acidity in pylorus-ligated rats; it did not affect basal acid secretion and the secretion stimulated by histamine, pentagastrin and 2-deoxy-D-glucose in the lumen-perfused stomach of anaesthetized rats, when administered by continuous infusion (0.01-1-mu-mol/kg/h) or bolus injection (0.05-25-mu-mol/kg). In this preparation, the H-3 agonist increased acid secretion at doses of 3-25-mu-mol/kg i.v., the effect being antagonized by famotidine. In the isolated gastric fundus from immature rats both (R)alpha-methylhistamine (0.01-10-mu-mol/l) and thioperamide (0.01-1-mu-mol/1) were totally ineffective against both spontaneous and stimulated gastric secretion. These results suggest that histamine H-3 receptors are unlikely to have a role in regulating gastric acid secretion in the rat.