Isolation of Mesenchymal Stem Cells from Adipose Tissue

被引：3

作者：

Harsan ^{[1
]}

Mariya, Silmi ^{[2
]}

Sajuti, Dondin ^{[2
]}

Islam, Andi Asadul ^{[3
]}

Wahjoepramono, Eka Julianta ^{[1
]}

Yusuf, Irawan ^{[3
]}

机构：

[1] Pelita Harapan Univ, Fac Med, Jl Siloam 6, Tangerang, Banten, Indonesia

[2] Bogor Agr Univ, Primate Res Ctr, Bogor, Indonesia

[3] Hasanudin Univ, Fac Med, Makassar, Indonesia

来源：

INDONESIAN BIOMEDICAL JOURNAL | 2015年 / 7卷 / 03期

关键词：

adipose; mesenchymal stem cells; isolation; immunophenotype;

D O I：

10.18585/inabj.v7i3.181

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

BACKGROUND: In searching for the best source of stem cells, researcher found adipose stem cells as one of the ideal source due to its easiness in harvesting and its potential for differentiating into other cell lineage. METHODS: We isolated stem cells from adipose tissue, cultured and conirmed its immunophenotype using polymerase chain reaction. RESULTS: Cluster of differentiation (CD) 44, CD7., CD90, CD105 were expressed, which represent immunophenotype of mesenchymal stem cells. CONCLUSION: Mesenchymal stem cells can be isolated from adipose tissue.

引用

页码：153 / 156

页数：4

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