CRYSTALLIZATION OF A PROFORM OF AEROLYSIN, A HOLE-FORMING TOXIN FROM AEROMONAS-HYDROPHILA

被引：20
|
作者：
TUCKER, AD [1 ]
PARKER, MW [1 ]
TSERNOGLOU, D [1 ]
BUCKLEY, JT [1 ]
机构：
[1] UNIV VICTORIA,DEPT BIOCHEM & MICROBIOL,VICTORIA V8W 2Y2,BC,CANADA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 212卷 / 04期
关键词：
D O I：
10.1016/0022-2836(90)90222-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Crystals of proaerolysin, the precursor of the hole-forming toxin from Aeromonas hydrophila, have been obtained. The mature form of this protein binds to a receptor on mammalian cells, aggregates and forms 30 Å holes in the membrane. The crystals are tetragonal, space group P43212, a = b = 104.00 A ̊, c = 222.0 A ̊. They contain a dimer in the asymmetric unit and diffract to a resolution of 2.6 Å. © 1990.
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收藏
页码：561 / 562
页数：2
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