CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF ISOCITRATE DEHYDROGENASE FROM THERMUS-THERMOPHILUS HB8

被引：3

作者：

OHZEKI, M ^{[1
]}

YAOI, T ^{[1
]}

MORIYAMA, H ^{[1
]}

OSHIMA, T ^{[1
]}

TANAKA, N ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,FAC BIOSCI & BIOTECHNOL,DEPT LIFE SCI,MIDORI KU,YOKOHAMA,KANAGAWA 226,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1995年 / 118卷 / 04期

关键词：

CRYSTALLIZATION; ISOCITRATE DEHYDROGENASE; SYNCHROTRON; THERMUS THERMOPHILUS;

D O I：

10.1093/oxfordjournals.jbchem.a124963

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant, Pillar-like crystals of about 0.6 X 0.6 X 0.3 mm(3) were obtained. Analysis of a series of oscillation photographs indicated that the orthorhombic crystals belonged to the I222 or I2(1)2(1)2(1) space group with unit cell dimensions of a=100.1 Angstrom, b=150.4 Angstrom, and c = 87.4 Angstrom. Intensity data were collected up to 2.5 Angstrom resolution.

引用

页码：679 / 680

页数：2

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