EXPRESSION OF HUMAN PLASMINOGEN-ACTIVATOR INHIBITOR-2 IN THE BACULOVIRUS EXPRESSION SYSTEM

被引：0
|
作者：
PEI, Y [1 ]
ZHOU, AW [1 ]
CAO, XR [1 ]
XU, XX [1 ]
机构：
[1] NANJING UNIV,NATL LAB PHARMACEUT BIOTECHNOL,NANJING 210008,PEOPLES R CHINA
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1995年 / 35卷 / 05期
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D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA coding for human plasminogen activator inhibitor-2 (PAI-2) was transfered into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus synthesized a 43-kDa PAI-2 protein, the majority of which was secreted. The recombinant PAI-2 was identical to native PAI-2 purified from human placenta with respect to interaction with polyclonal antibody and inhibition of urokinase-type plasminogen activator.
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页码：923 / 928
页数：6
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