PRODUCING A BACTERIOPHAGE-P1 LIBRARY FROM PINE - ISOLATION AND CLONING OF VERY HIGH-MOLECULAR-WEIGHT DNA

被引：0
|
作者：
GORMAN, SW
ROBERTSOEHLSCHLAGER, SL
CULLIS, CA
TEASDALE, RD
机构：
[1] BOND UNIV,SOUTHPORT,AUSTRALIA
[2] CASE WESTERN RESERVE UNIV,CLEVELAND,OH 44106
来源：
BIOTECHNIQUES | 1992年 / 12卷 / 05期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have generated a genomic P1 bacteriophage library using Monterey pine (Pinus radiata) DNA. We first developed a method for isolating from pine tissue the very high molecular weight DNA necessary for the preparation of libraries requiring large inserts. The method involves protoplasting the cells, isolating nuclei and lysis in a high concentration of detergent. Fragments of greater than two megabases in size are produced in solution. Modi cations introduced to the protocol for library preparation and for P1 plasmid isolation are described.
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页码：722 / &
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