SYMPATHOADRENAL AND OTHER ENDOCRINE AND METABOLIC RESPONSES TO HYPOGLYCEMIA IN THE FETAL FOAL DURING LATE-GESTATION

In the present study, ten insulin challenge tests were carried out on nine chronically catheterized fetal foals between 253 and 314 days gestation (term > 320 days). Changes in fetal plasma concentrations of glucose, catecholamines, cortisol, ACTH, free fatty acid (FFA) and lactate were measured before and after a bolus dose of insulin (0.5-2.0 u/kg I.V.). Fetal blood gases, pH, haemoglobin levels and heart rate were measured throughout the 2-3 h experimental period. The fetuses fell into two distinct groups on the basis of proximity to delivery and basal plasma cortisol and catecholamine levels. Those within 2-10 days of delivery after 300 days (group 2, number of experiments (n) = 4) had plasma cortisol and noradrenaline concentrations which were significantly higher (P < 0.05) than the corresponding values in fetuses sampled earlier in gestation or at least 2 weeks before foaling (group 1, n = 6). Although insulin administration resulted in a 50% fall in plasma glucose in all animals, group 2 showed significantly greater increases in plasma noradrenaline than group 1. In neither group were there detectable changes in plasma adrenaline. In group 2, increases in plasma cortisol were seen following insulin, whereas no cortisol changes were observed in group 1, despite rises in plasma ACTH. Insulin administration also led to acidosis and increases in heart rate and plasma lactate and FFA levels in all fetuses studied. However, only group 2 became consistently hypoxic during the insulin challenge. No significant changes in plasma glucose or any of the other parameters were observed in the fetuses after saline administration (n = 5). These findings suggest that hypoglycaemia activates the sympathoadrenal system in the fetal foal from 75% of gestation but has relatively little effect on the fetal pituitary-adrenocortical axis until much closer to term.