ULTRASTRUCTURAL ORGANIZATION OF THE NORADRENERGIC INNERVATION OF THE SUPERFICIAL GRAY LAYER OF THE HAMSTERS SUPERIOR COLLICULUS

Immunocytochemistry with an antibody-directed tyrosine hydroxylase (TH) was combined with electron microscopy and serial-section analysis to examine the synaptic organization of the catecholaminergic projection to the stratum griseum superficiale (SGS) of the hamster's superior colliculus (SC). A total of 250 TH-immunoreactive profiles within SGS were examined. Of these, 114 (45.6%) made synaptic contacts; 81 (71.1%) were axodendritic, and the remainder (33, 28.9%) were axo-axonic. Serial-section analysis was employed to evaluate the presence or absence of synaptic contacts for 26 profiles. Overall, 19 (73.1%) of the profiles followed through serial sections exhibited synaptic contacts. Double staining of single sections with antibodies directed against TH and dopamine-beta-hydroxylase (D beta H) and examination in the light microscope indicated that virtually all TH-positive fibers also contained D beta H. This indicated that the fibers examined at the electron microscopic level were noradrenergic rather than dopaminergic. These results suggest that norepinephrine may have both pre- and postsynaptic actions in the hamster's SC and that at least some of these effects are mediated by conventional synapses. (C) 1994 Wiley-Liss, Inc.