OXIDIZED AND REDUCED GLUTATHIONE, ASCORBATE AND GLUTATHIONE-REDUCTASE IN MATRICARIA-RECUTITA L CALLUS-CULTURES

In the long-term cultivated callus cultures of Matricaria recutita L. the identical concentration changes in the biosynthesis of glutathione, glutamate, aspartate, total thiols and proteins were detected within the subculture. The level of oxidized glutathione during the growth of callus culture was low with the highest value 10.66 nmol g-1 on the 13th day of subculture. The ratio GSH/GSSG which significantly influences the redox processes in a cell, and the activity of glutathione reductase increased from the 8th day. Ascorbate formation was detected on the 17th day, although no relation between the ascorbate synthesis and the concentration of glutathione and glutathione reductase was found. © 1990 Kluwer Academic Publishers.