EXPERIMENTAL-INFECTION OF PIGS WITH A PARVOVIRUS ISOLATED FROM THE DIARRHEIC FECES OF A PIG

A parvovirus (the H-45 strain) isolated from an outbreak of epizootic diarrhea in swine was examined to observe the infectivity and pathogenecity in swine. The virus infection by intranasal route was demonstrated in each group of 2- and 5-day-old colostrum-deprived pigs, 30- and 100-day-old pigs by virus recovery from the nasal and rectal swabs, and detecting seroconversion. The virus was recovered from rectal swabs up to 14 days after inoculation and from nasal swabs up to 9 days. Uninoculated pigs were infected with the virus by contacting with the inoculated pigs. Between 1 and 5 days after inoculation, the inoculated pigs of 2, 5 and 30 days old developed diarrhea and then all the pigs of 2 days old died, resulting from dehydration. In the pigs died after intranasal inoculation, the virus was recovered mainly from the respiratory and gastrointestinal tracts. One hundred-day-old pigs showed no clinical signs. The pathological change was characterised by congestion and edema with hemorrhage in the epithelium of the small intestines exhibiting additional degeneration ana desquamation of the epithelial cells.