Efficiency of three Pseudomonas isolates in releasing phosphate from an artificial variable-charge mineral (iron III hydroxide)

被引:0
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作者
Ghaderi, A. [1 ]
Aliasgharzad, N. [1 ]
Oustan, S. [1 ]
Olsson, P. A. [2 ]
机构
[1] Univ Tabriz, Fac Agr, Dept Soil Sci, Tabriz, IR, Iran
[2] Lund Univ, Dept Ecol, Plant Ecol & Systemat, Lund, Sweden
关键词
Pseudomonas; phosphate releasing; variable-charge minerals; specific P adsorption;
D O I
暂无
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Phosphorus management in agriculture involves acquiring exact and comprehensive information about the fate of added phosphate to the soil. A great part of a phosphate (P) fertiliser is retained on the surface of variable charge minerals through specific adsorption; the precipitation process takes place in the limited space around the fertiliser grains where its concentration is high. We tested the ability of three Pseudomonas strains to release P frim iron (III) hydroxide. Iron hydroxide precipitated on filter paper, was used a variable-charge mineral and P was loaded on it. The siderophore and H+ production, acid and alkaline phosphatases activity were assayed in vitro. All three isolates released P as compared to controls with killed bacteria or without bacteria. The amount of released P from iron hydroxide were 50.5, 29.2 and 61.6% for P. putida, P.fluorescens Chao and P. fluorescens Tabriz, respectively. The amount of P in solution phase was highest (0.74 mg P/50mL) with P. putida and lowest (0.32 mg P/50 mL) with P. fluorescens Tabriz The highest P assimilation from total P released was in P. fluorescens Tabriz. In vitro tests revealed that the P. fluorescens Chao with significantly higher siderophore production and phosphatases activity, had the least effect on P release from iron hydroxide, while two other isolates had significantly higher H+ production is the main mechanism of P released from variable charge minerals and P.putida was the most efficient bacterium.
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页码:71 / 76
页数:6
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