ENHANCEMENT OF CA2+ RELEASE CHANNEL ACTIVITY BY PHOSPHORYLATION OF THE SKELETAL-MUSCLE RYANODINE RECEPTOR

被引：68
|
作者：
HERRMANNFRANK, A
VARSANYI, M
机构：
[1] RUHR UNIV BOCHUM, INST PHYSIOL CHEM, BIOCHEM SUPRAMOLEK SYST ABT, D-44780 BOCHUM, GERMANY
[2] UNIV ULM, ANGEW PHYSIOL ABT, D-89069 ULM, GERMANY
来源：
FEBS LETTERS | 1993年 / 332卷 / 03期
关键词：
SKELETAL MUSCLE; RYANODINE RECEPTOR; PHOSPHORYLATION; CA2+-CHANNEL; SARCOPLASMIC RETICULUM; CA2+ RELEASE;
D O I：
10.1016/0014-5793(93)80640-G
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Ca2+ release channel of rabbit skeletal muscle sarcoplasmic reticulum (SR) can be phosphorylated by membrane associated protein kinase(s) utilizing endogenously synthesized or exogenously added ATP. The channel protein has been enriched in non-phosphorylated and phosphorylated form from heavy SR following solubilization with CHAPS (3-[(3-cholamidopropyl)dimethylammonio-1-propane-sulfonate) and ultracentrifugation on a linear sucrose/CHAPS gradient. Reconstitution of the isolated channels into planar bilayers shows that phosphorylation enhances the open probability by increasing the sensitivity towards micromolar Ca2+ and ATP. The phosphorylation induced enhancement of the channel activity can be reversed by purified protein phosphatase 2A.
引用
收藏
页码：237 / 242
页数：6
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