GENETIC DIVERSITY STUDY OF CLUSTER BEAN (Cyamopsis tetragonoloba (L.) Taub) LANDRACES USING RAPD AND ISSR MARKERS

The main source of genetic variability for breeding and crop improvement in cluster bean is landraces. Genetic diversity in 29 landraces and 19 commercial varieties of cluster bean belonging to Gujarat, Rajasthan, Haryana and Delhi regions of India were analyzed in the present study using 13 RAPD (Randomly Amplified Polymorphic DNA) and 7 ISSR (Inter Simple Sequence Repeat) markers. Amplification with RAPD primers produced a total of 118 bands, out of which 103 were polymorphic. Out of the 13 primers used, OPQ-09 produced the highest number of bands (12). The average percentage polymorphism for RAPD markers was 87.63. Seven ISSR markers produced 64 bands out of which 50 were polymorphic. Among the ISSR primers used UBC-868 produced highest number of bands (13); the average percentage polymorphism for ISSR markers was 77.82. UPGMA tree was constructed using Jaccard's similarity for RAPD, ISSR and RAPD+ISSR data. RAPD and RAPD+ISSR showed separate clustering of landraces from commercial varieties. AMOVA showed the presence of higher variance within the populations than between the populations. The results obtained from the present study can be used for selecting accessions for breeding and crop improvement in cluster bean.