SHEEP;
HAEMONCHUS CONTORTUS;
ELISA;
WESTERN BLOTTING;
ANTIGEN;
BODY FRAGMENTS;
ESOPHAGUS;
D O I:
10.1016/0020-7519(95)99844-9
中图分类号:
R38 [医学寄生虫学];
Q [生物科学];
学科分类号:
07 ;
0710 ;
09 ;
100103 ;
摘要:
Adult specimens of Haemonchus contortus were microdissected into four body fragments: oesophagus, cuticle of oesophagus, gut and cuticle with adjacent muscle layer. The antigenicity of these different body fragments was analysed in comparison to total (whole) worm extracts with immunoblotting and ELISA using sera of H. contortus-infected sheep. Tn particular, oesophagus-derived antigens appeared to be specifically recognized and may prove valuable in diagnostic assays.