CROSS-SPECIES AMPLIFICATION OF MICROSATELLITE LOCI IN THREE MANGROVE SPECIES OF INDIA

被引：0
|
作者：
Kumar, B. Ashish [1 ]
Thangaraj, M. [1 ]
Kathiresan, K. [1 ]
机构：
[1] Annamalai Univ Parangipettai, Ctr Adv Study Marine Biol, Parangipettai 608502, India
来源：
INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH | 2011年 / 2卷 / 02期
关键词：
Mangrove; Rhizopora; Microsatellite; Genetic variation;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study analysed genetic relationship in three species of Rhizophora growing in southeast coast of India. Eight microsatellite loci in three mangrove species (Rhizophora annamalayana, R. apiculata and R. mucronata) were successfully amplified by cross priming. All the loci were polymorphic and ideal to use as markers in identification of population. However, the optimum annealing temperature to get scorable band in Rhizophora species varied from that reported in literature for the respective primer pair. Sixteen alleles were observed in eight loci. The number of alleles for overall species was 0.3333 per locus and for overall locus was 0.2619 per species. Rhizophora annamalayana and R. mucronata were found genetially much closer (0.006) than R. apiculata (0.008) as revealed by microsatellite data.
引用
收藏
页码：240 / 243
页数：4
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