PRODUCTION AND FUSION OF PROTOPLASTS OF FUNGUS PRODUCERS OF LIGNOLYTIC AND CELLULOLYTIC ENZYMES - BIOCHEMICAL CHARACTERISTICS OF THE FUSANT ENZYME COMPLEX

被引：0

作者：

OBRAZTSOVA, IN ^{[1
]}

NUTSUBIDZE, NN ^{[1
]}

PRABAKARAN, K ^{[1
]}

DEMINA, VA ^{[1
]}

GERNET, MV ^{[1
]}

机构：

[1] ACAD SCI GESSR,INST PLANT BIOCHEM,TBILISI,GEORGIA,USSR

来源：

BIOCHEMISTRY-MOSCOW | 1991年 / 56卷 / 06期

关键词：

FUNGUS PROTOPLASTS; FUSION OF PROTOPLASTS; LIGNOLYTIC ACTIVITY; CELLULOLYTIC ACTIVITY;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intergeneric fusion of protoplasts of fungi belonging to taxonomically distant genera, formally even to different classes - Deuteromycetes (Trichoderma longibrachiatum) and Basidiomycetes (Phanerochaete chrysosporium) - has been accomplished. It was shown that the fusant strain A 2-80 secretes an enzyme complex balanced for cellulolytic and lignolytic activities. The identification was performed according to the morphological properties, the rate of agglutination of the conidia by lectins, enzyme activities, and biochemical characteristics of the original parental and fusant strains. The microorganism A 2-80 produced by protoplast fusion is viable, stable, and does not break down to the original parental strains in a period of more than two years, withstanding at least 60 reinoculations. The ratio of the lignolytic and cellulolytic activities was found to be virtually invariant during the investigation.

引用

页码：699 / 704

页数：6