EXTRACELLULAR DEPOLYMERIZATION OF HYALURONIC-ACID IN CULTURED HUMAN SKIN FIBROBLASTS

被引：31

作者：

NAKAMURA, T ^{[1
]}

TAKAGAKI, K ^{[1
]}

KUBO, K ^{[1
]}

MORIKAWA, A ^{[1
]}

TAMURA, S ^{[1
]}

ENDO, M ^{[1
]}

机构：

[1] HIROSAKI UNIV,SCH MED,DEPT BIOCHEM,5 ZAIFU CHO,HIROSAKI,AOMORI 036,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)80174-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chain length of [3H]hyaluronic acid synthesized by cultivating human skin fibroblasts in the presence of [3H]glucosamine was investigated. [3H]Hyaluronic acid obtained from the matrix fraction was excluded from a Sepharose CL-2B column irrespective of the incubation period, whereas that from the medium was depolymerized into a constant chain length (Mr=40, 000). The reducing and non-reducing terminals of the depolymerized hyaluronic acid were N-acetylglucosamine and glucuronic acid, respectively. Prolonged incubation produced no oligosaccharides as shown by examination of hyaluronidase digests, suggesting the presence of a novel endo-β-N-acetylglucosaminidase in cultured human skin fibroblasts. © 1990 Academic Press, Inc.

引用

页码：70 / 76

页数：7

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