STUDIES ON PHOSPHOLIPASE-A2 IN HUMAN SEMINAL PLASMA

被引：10
|
作者：
RONKKO, S
RASANEN, M
机构：
来源：
INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1992年 / 24卷 / 06期
关键词：
D O I：
10.1016/0020-711X(92)90108-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. Human seminal plasma and posterior lobe of prostate was found to have phospholipase A, (PLA2) activity hydrolysing phosphatidylethanolamine with C-14-labelled linoleic and arachidonic acid. 2. A negative relationship was between sperm count and PLA2 activity in human seminal plasma. 3. The purified PLA2 from human seminal plasma showed high affinity to heparin, sensitivity toward p-bromophenacyl bromide, Pb2+, dithioerythritol and EDTA and it was activated by Ca2+ and Mn2+. 4. The purified PLA2 had alkaline pH optimum (7.5-10.0) and pI-value of 5.3. In SDS-PAGE enzyme preparation resulted in two bands with mol. wt of 14,000 and 16,000.
引用
收藏
页码：987 / 992
页数：6
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