MONOCLONAL-ANTIBODIES THAT DETECT LIVE SALMONELLAE

被引：14
|
作者：
TORENSMA, R
VISSER, MJC
AARSMAN, CJM
POPPELIER, MJJG
VANBEURDEN, R
FLUIT, AC
VERHOEF, J
机构：
[1] U GENE RES BV,3584 CS UTRECHT,NETHERLANDS
[2] LUMAC BV,6370 AC LANDRAAF,NETHERLANDS
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 12期
关键词：
D O I：
10.1128/AEM.58.12.3868-3872.1992
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were reactive with serogroups A, B, C1, C2, D, E, and K and Salmonella choleraesuis subsp. diarizonae. Each monoclonal antibody proved to be reactive with a distinct serotype. Clinical isolates belonging to these Salmonella serogroups could be detected. Reactivity with non-Salmonella bacteria proved to be minor.
引用
收藏
页码：3868 / 3872
页数：5
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