DELETION MUTAGENESIS IN M13 BY POLYMERASE CHAIN-REACTION USING UNIVERSAL SEQUENCING PRIMERS

被引：3

作者：

TANNICH, E ^{[1
]}

TUMMLER, M ^{[1
]}

ARNOLD, HH ^{[1
]}

LINGELBACH, K ^{[1
]}

机构：

[1] BERNHARD NOCHT INST TROP MED,HAMBURG,GERMANY

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 188卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90602-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple procedure is described for the efficient deletion of large DNA sequences. The method involves a combination of oligonucleotide-directed mutagenesis in bacteriophage M13 and amplification of the mutagenized product by polymerase chain reaction. In contrast to other protocols employing polymerase chain reaction, synthesis of only one specific primer is required. The efficiency of heteroduplex formation between mutagenic primers directing large deletions and singlestranded template is discussed. © 1990.

引用

页码：255 / 258

页数：4

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