P-32 POSTLABELING ANALYSIS OF DNA FROM HUMAN TISSUES

被引：27
|
作者：
ROUTLEDGE, MN
GARNER, RC
JENKINS, D
CUZICK, J
机构：
[1] UNIV YORK,DEPT BIOL,JACK BIRCH UNIT ENVIRONM CARCINOGENESIS,YORK YO1 5DD,N YORKSHIRE,ENGLAND
[2] WHITTINGTON HOSP,LONDON N19 5NF,ENGLAND
[3] IMPERIAL CANC RES FUND,LONDON WC2A 3PX,ENGLAND
来源：
MUTATION RESEARCH | 1992年 / 282卷 / 03期
关键词：
DNA; P-32-POSTLABELING ANALYSIS; NUCLEASE P1 ENHANCEMENT; P-32-POSTLABELING ASSAY;
D O I：
10.1016/0165-7992(92)90087-X
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
DNA extracted from human lung, bladder, liver, pancreas, cervix and breast tissue samples taken at autopsy (37 sample sets) was analysed by the nuclease P1 enhancement modification of the P-32-postlabelling assay for levels of aromatic carcinogen DNA adducts. Results were combined with those from a previous study for statistical analysis of 56 sample sets (32 male + 24 female). A strong trend was seen for increased adduct levels in the lung DNA of smokers and a weak association for the bladder DNA of smokers compared to non-smokers. Aromatic adducts were also detected in other tissues.
引用
收藏
页码：139 / 145
页数：7
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