PECULIAR PROPERTIES OF LIPASE FROM CANDIDA-PARAPSILOSIS (ASHFORD) LANGERON AND TALICE

被引：22
|
作者：
RIAUBLANC, A
RATOMAHENINA, R
GALZY, P
NICOLAS, M
机构：
[1] INRA,ENSA,CHAIRE MICROBIOL IND & GENET MICROORGANISMES,PL VIALA,F-34060 MONTPELLIER 1,FRANCE
[2] INST BIOL,INSERM,U65,F-34000 MONTPELLIER,FRANCE
来源：
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1993年 / 70卷 / 05期
关键词：
CANDIDA-PARAPSILOSIS; 1,3 DIGLYCERIDES; ESTERASE; ESTERS; LIPASE; LIPIDS; MONOGLYCERIDES; SPECIFICITY; SYNTHESIS;
D O I：
10.1007/BF02542583
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A new Candida parapsilosis lipase was isolated and studied. This enzyme was purified by hydrophobic chromatography on a phenyl-sepharose CL4B column followed by gel permeation on a Sephacryl S300 HR column. It was a 160 kg.mol-1 molecular-weight oligomeric enzyme. Optimal activity was obtained at 45-degrees-C and pH 6.5. The lipase activity toward various acylglycerols and esters was studied. The hydrolysis rate was greater for secondary acylesters than for primary acylesters. This lipase showed a high specificity for long-chain fatty acids and particularly for polyunsaturated fatty acids. This enzyme was able to catalyze the synthesis of various oleoylesters in aqueous medium.
引用
收藏
页码：497 / 500
页数：4
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