LIQUID-CHROMATOGRAPHIC REACTION PROCESS OF THE ENZYMATIC-HYDROLYSIS OF STARCH

Microporous spherical glass in a liquidchromatographic reactor is coated dynamically by alpha-amylase. The development of fixation of the enzyme can be followed, if pulses of starch are introduced into the reactor at definite times. The shape of measured reaction chromatograms remains constant if a stationary state is achieved. Volume-velocity, concentration of enzyme and the temperature of the reactor have influence on the development of stationary coating conditions and the progress of starch hydrolysis. Experimental rate constants can be obtained by means of reaction chromatograms.