The conformation of tripler DNA d(CT)(8) . d(AG)(8) . d(C+T)(8) in solution has been investigated by near infrared FT-Raman spectroscopy at 1064 nm, Results clearly revealed that almost all of the sugar pukers of the tripler fall in the normal range for the B-genus, However, a strong band at 1266 cm(-1) suggested that the C3'-endo/anti protonated cytosines in the third strand can not be eliminated.