MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A CDNA-ENCODING A HUMAN PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE RECEPTOR

被引：107
|
作者：
OGI, K [1 ]
MIYAMOTO, Y [1 ]
MASUDA, Y [1 ]
HABATA, Y [1 ]
HOSOYA, M [1 ]
OHTAKI, T [1 ]
MASUO, Y [1 ]
ONDA, H [1 ]
FUJINO, M [1 ]
机构：
[1] TAKEDA CHEM IND LTD,DISCOVERY RES LABS,10 WADAI,TSUKUBA,IBARAKI 30042,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 196卷 / 03期
关键词：
D O I：
10.1006/bbrc.1993.2423
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A functional cDNA clone for a human pituitary adenylate cyclase activating polypeptide (PACAP) receptor was isolated from a human pituitary cDNA library. The cDNA encoded a polypeptide consisting of 525 amino acids with putative seven hydrophobic domains. Chinese hamster ovary (CHO)-K1 cells transfected with the cDNA specifically bound PACAP and mediated PACAP-triggered intracellular accumulation of cAMP, indicating that this cDNA encoded a functional human PACAP Type I receptor. This receptor was structurally related to the vasoactive intestinal peptide (VIP), secretin, calcitonin and parathyroid hormone receptors and is much more homologous to a rat PACAP receptor. Northern blot analysis revealed that PACAP receptor mRNAs were expressed mainly in the brain and widely distributed in the central nervous system. © 1993 Acdamic Press. Inc.
引用
收藏
页码：1511 / 1521
页数：11
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