ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE DETECTION OF PORCINE EPIDEMIC DIARRHEA CORONAVIRUS ANTIBODIES IN SWINE SERA

被引：31
|
作者：
HOFMANN, M [1 ]
WYLER, R [1 ]
机构：
[1] UNIV ZURICH,INST VIROL,WINTERTHURERSTR 266A,CH-8057 ZURICH,SWITZERLAND
来源：
VETERINARY MICROBIOLOGY | 1990年 / 21卷 / 03期
关键词：
D O I：
10.1016/0378-1135(90)90037-V
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushion proved to be the best antigen purification method. Examination of 1024 swine sera showed a high specificity and a greater sensitivity of the ELISA, when compared with indirect immunofluorescence. Reference sera with high antibody titers to PEDV originated from two pigs experimentally infected with PEDV. Three different antigen purification methods and the advantages of the ELISA compared with an immunofluorescence test are discussed. © 1990.
引用
收藏
页码：263 / 273
页数：11
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