ALIGNING HISTOLOGICAL SERIAL SECTIONS FOR 3-DIMENSIONAL RECONSTRUCTION USING AN EXCIMER-LASER BEAM

被引:8
|
作者
CLARKE, GE
HAMILTON, PW
MONTGOMERY, WA
机构
[1] ROYAL VICTORIA HOSP,DEPT PATHOL,QUANTITAT PATHOL LAB,BELFAST BT12 6BL,NORTH IRELAND
[2] QUEENS UNIV BELFAST,DEPT PHYS,BELFAST BT7 1NN,ANTRIM,NORTH IRELAND
关键词
COLORECTUM; EXCIMER LASER; VIDAS IMAGE ANALYSIS;
D O I
10.1016/S0344-0338(11)80367-5
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Accurate alignment of histological sections has always been a fundamental problem in the three-dimensional reconstruction of tissue structures from serial sections. An excimer laser was used to create small boles in paraffin processed colorectal tissue blocks. The laser holes were 200-230 mum in diameter and penetrated the tissue approximately 0.5 mm. No damage was caused to the surrounding tissue. Using a VIDAS image analyser, these microscopic holes could be used as artificial reference points (ARPs) for the accurate alignment of consecutive serial sections and three-dimensional reconstruction of colorectal glandular tissue.
引用
收藏
页码:563 / 566
页数:4
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